PCR Exercise 1. Match the words with their definitions
Amplification
Complementary
Anneal
Denaturation
Archaea
Dissociation
Exploit
Facilitate
Flank
Impact
nucleic acid
primer
probe
strand
substrate
template
yield
A. A molecule or surface on which a reaction occurs- S _______________
B. To make a process easier
C. The separation of components that were previously connected
D. A short nucleic acid sequence used to initiate replication
E. To increase the amount or strength of something
F. A single thread-like structure
G. A pattern used as a guide for producing something else
H. The influence or effect of one thing on another
I. A group of ancient single-celled microorganisms
J. To attach or bind together
K. A molecule used to detect the presence of another molecule
L. DNA or RNA
M. Positioned on either side of something
N. The amount produced by a process
O. Having parts that complete or enhance each other
P. The loss of a molecule's natural structure
Q. To make use of something, often for a particular purpose
Exercise 2. Complete the sentences
The researchers used a fluorescent............. to identify the target DNA sequence.
The two DNA strands are .............. to one another.
Heating the sample caused ............... of the protein.
Scientists developed a new method to ................. data analysis.
During PCR, the primers .................... to the target sequence.
The company hopes to ................. advances in biotechnology.
The reaction produced a higher ................ than expected.
amplification • complementary • anneal • denaturation • exploit • facilitate • flank • impact • primer • probe • substrate • template • yield
The enzyme could not function because the .......... was absent.
The mountains .................. the valley on both sides.
The environmental ................. of the project must be assessed.
DNA ................... is a crucial step in PCR.
The new strand is synthesized using a DNA..............
A .................... is required to begin DNA replication.